马来酰亚胺染料连接带巯基的蛋白或生物分子
马来酰亚胺染料连接带巯基的蛋白或生物分子
马来酰亚胺活性基团的荧光染料是目前和带有巯基的生物分子比如说多肽、蛋白或者小分子做标记或者偶联的最常用的试剂。
maleimides是一个对thiols有高选择性的亲电化合物，在自然界中基本上不存在maleimides基团，而有丰富的thiols存在，马来酰亚胺和巯基会作为半胱氨酸残基在多肽和蛋白中相遇，虽然天然的dna中不含有巯基，但是人工合成的寡核苷酸是非常容易制备出来的。
cy3 maleimides , cy5 maleimides , cy7 maleimides标记带有sh的多肽、蛋白还有生物小分子的具体实验方法和实验步骤。
1. dissolve the protein or other moleculecontaining thiol to be labeled in degassed buffer (pbs, tris, hepes are good,although others buffers containing no thiols can be used) at ph 7-7.5 inplastic vial. buffer can be degassed by applying vacuum on it for severalminutes, or by bubbling through inert gas (nitrogen, argon, or helium). forproteins, good concentration is between 1-10 mg/ml.
2. add an excess of tcep(tris-carboxyethylphosphine) reagent to reduce disulfide bonds, flush withinert gas, and close. 100x molar excess of tcep is fine. keep the mixture for20 minutes at room temperature.
3. dissolve maleimide in dmso or fresh dmf(1-10 mg in 100 ul).
4. add dye solution to thiol solution (20xfold excess of dye), flush vial with inert gas, and close tightly. 5. mixthoroughly, and keep overnight at room temperature, or 4 celsius. 6. purify bygel filteration, hplc, fplc, or electrophoresis
for maleimides with poor aqueoussolubility, like most dye maleimides, we recommend use of cosolvent (dmf ordmso). maleimides with good aqueous solubility (like sulfo-cy maleimides) canbe dissolved in water. if precipitation occurs, increase content of organicco-solvent in the mixture to achieve better labeling.
相关试剂信息：
cyanine3maleimide
appearance:
red powder
molecular weight:
615.20
molecular formula:
c36h43cln4o3
solubility:
soluble in organic solvents (dmf, dmso, dichloromethane), insoluble in water
quality control:
nmr 1h (95%) and 13c, tlc, functional testing
storage conditions:
storage: 24 months at -20°c in the dark.
cyanine5maleimide
appearance:
dark blue powder
molecular weight:
641.24
molecular formula:
c38h45cln4o3
solubility:
soluble in organic solvents (dmf, dmso, dichloromethane), insoluble in water
quality control:
nmr 1h (95%) and 13c, tlc, functional testing
storage conditions:
storage: 24 months at -20°c in the dark
cyanine7maleimide
appearance:
green powder
molecular weight:
707.34
molecular formula:
c43h51cln4o3
solubility:
soluble in organic solvents (dmso, dmf, dichloromethane), low solubility in water
quality control:
nmr 1h (95%) and 13c, tlc, functional testing
storage conditions:
storage: 24 months at -20°c in the dark.
以上资料源于西安瑞禧生物科技有限公司
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马来酰亚胺活性基团的荧光染料是目前和带有巯基的生物分子比如说多肽、蛋白或者小分子做标记或者偶联的最常用的试剂。
maleimides是一个对thiols有高选择性的亲电化合物，在自然界中基本上不存在maleimides基团，而有丰富的thiols存在，马来酰亚胺和巯基会作为半胱氨酸残基在多肽和蛋白中相遇，虽然天然的dna中不含有巯基，但是人工合成的寡核苷酸是非常容易制备出来的。
cy3 maleimides , cy5 maleimides , cy7 maleimides标记带有sh的多肽、蛋白还有生物小分子的具体实验方法和实验步骤。
1. dissolve the protein or other moleculecontaining thiol to be labeled in degassed buffer (pbs, tris, hepes are good,although others buffers containing no thiols can be used) at ph 7-7.5 inplastic vial. buffer can be degassed by applying vacuum on it for severalminutes, or by bubbling through inert gas (nitrogen, argon, or helium). forproteins, good concentration is between 1-10 mg/ml.
2. add an excess of tcep(tris-carboxyethylphosphine) reagent to reduce disulfide bonds, flush withinert gas, and close. 100x molar excess of tcep is fine. keep the mixture for20 minutes at room temperature.
3. dissolve maleimide in dmso or fresh dmf(1-10 mg in 100 ul).
4. add dye solution to thiol solution (20xfold excess of dye), flush vial with inert gas, and close tightly. 5. mixthoroughly, and keep overnight at room temperature, or 4 celsius. 6. purify bygel filteration, hplc, fplc, or electrophoresis
for maleimides with poor aqueoussolubility, like most dye maleimides, we recommend use of cosolvent (dmf ordmso). maleimides with good aqueous solubility (like sulfo-cy maleimides) canbe dissolved in water. if precipitation occurs, increase content of organicco-solvent in the mixture to achieve better labeling.
相关试剂信息：
cyanine3maleimide
appearance:
red powder
molecular weight:
615.20
molecular formula:
c36h43cln4o3
solubility:
soluble in organic solvents (dmf, dmso, dichloromethane), insoluble in water
quality control:
nmr 1h (95%) and 13c, tlc, functional testing
storage conditions:
storage: 24 months at -20°c in the dark.
cyanine5maleimide
appearance:
dark blue powder
molecular weight:
641.24
molecular formula:
c38h45cln4o3
solubility:
soluble in organic solvents (dmf, dmso, dichloromethane), insoluble in water
quality control:
nmr 1h (95%) and 13c, tlc, functional testing
storage conditions:
storage: 24 months at -20°c in the dark
cyanine7maleimide
appearance:
green powder
molecular weight:
707.34
molecular formula:
c43h51cln4o3
solubility:
soluble in organic solvents (dmso, dmf, dichloromethane), low solubility in water
quality control:
nmr 1h (95%) and 13c, tlc, functional testing
storage conditions:
storage: 24 months at -20°c in the dark.
以上资料源于西安瑞禧生物科技有限公司
东城区附近装饰工程公司更受欢迎
养马护栏，内蒙古草原围栏，PVC美式栅栏，马场防护
宝宝睡眠不足怎么办
城隍庙展柜制作哪家好|展柜制作
气动单轨吊 矿用运输单轨吊
马来酰亚胺染料连接带巯基的蛋白或生物分子
喜大普奔！月经假、岗位补贴、二胎补贴！更多利好政策源源不断！
预制聚氨酯发泡保温管价格
供应粉末尿素 化妆品级别 高纯度高保湿性能
到底我们的猪场该做哪些疫苗？绝对长知识！
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复联四：浩克觉醒后不仅穿上了黑背心，还带上了机械手套吊打灭霸
供应品牌 蒙娜丽莎 卫浴座便器/马桶
一分钟解读为什么不选平台电商
供应韩国OD-TECH齐纳二极管芯片
供应铝粉专用振动筛-防爆超声波振动筛（图）
北京主持人培训哪家比较好，行业领先
党旗党建宣传不锈钢玻璃钢雕塑广场大型环境房地产景观园林小品
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河南炼油设备生产厂家